Thermal Stabilization of an Endoglucanase by Cyclization
نویسندگان
چکیده
منابع مشابه
Thermal Stabilization of an Endoglucanase by Cyclization
An intein-driven protein splicing approach allowed for the covalent linkage between the N- and C-termini of a polypeptide chain to create circular variants of the endo-β-1,3-1,4-glucanase, LicA, from Bacillus licheniformis. Two circular variants, LicA-C1 and LicA-C2, which have connecting loops of 20 and 14 amino acids, respectively, showed catalytic activities that are approximately two and th...
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15 صفحه اولStabilization of a binary protein complex by intein-mediated cyclization.
The study of protein-protein interactions can be hampered by the instability of one or more of the protein complex components. In this study, we showed that intein-mediated cyclization can be used to engineer an artificial intramolecular cyclic protein complex between two interacting proteins: the largely unstable LIM-only protein 4 (LMO4) and an unstructured domain of LIM domain binding protei...
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Stabilization of an enzyme while maintaining its activity has been a major challenge in protein chemistry. Although it is difficult to simultaneously improve stability and activity of a protein by amino acid substitutions due to the activity-stability trade-off, backbone cyclization by connecting the N and C termini with a linker is promising as a general method of stabilizing a protein without...
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ژورنال
عنوان ژورنال: Applied Biochemistry and Biotechnology
سال: 2012
ISSN: 0273-2289,1559-0291
DOI: 10.1007/s12010-012-9674-z